Composite

Part:BBa_K1081000:Design

Designed by: Hangxing Jia   Group: iGEM13_WHU-China   (2013-09-10)


J13002-dcas9


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1175
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 3454
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We have to add prefix and suffix to both end of dcas9, so we add dcas9 to the plasmid by gibson assembly.

Source

 dCas9 was provided by addgene, which is a modified gene originated from Streptococcus pyogenes.

References

Repurposing CRISPR as an RNA-Guided Platform for Sequence-Specific Control of Gene Expression. Cell, 152, 1173-1183.